Spire : a new Rab27a-effector Characterizing Rab27a interaction-sites within spire is going to present by Noura Alzahofi(YRF)

Conf name: COD accredited 2nd International Conference on Molecular Biology and Stem Cells
Short Name: Molecular Biology 2019
Venue: June 06-07, 2019 at London, UK
URL: https://goo.gl/Ew42oj

Rab27a is a small GTPase and a member of the RAS oncogene family. Rab27a governs different Kinds of intracellular trafficking through interaction with distinct effectors. The extent of known Rab effectors has contributed to enlightening the molecular mechanism and dysfunctions that lead to a variety of human diseases. Recently, we identify spire, an actin nucleation protein, as a new Rab-27a effector. Spire interacts with formin-1 (an actin elongation factor) to nucleate linear actin-filaments that are used as a track for myosin to transport intracellular cargo, including melanosome in skin melanocytes. Using melanosome distribution as an indicator, in modified-nanoscale pulldowns assay, we found that spire is able to interact with Rab-27a on the cytoplasmic face of the melanosome via its C-terminal membrane-binding region. In addition, the results highlight a crucial role of Spire-Box domain in this interaction. Interestingly, a point mutation within Spire-Box (K419W) blocked the said interaction. This mutation corresponding to that of R35W in melanophilin/Slac2 (Rab27a-effector) that causes Griscelli syndrome type-3 in humans.

Comprehensive organic identification of biological particles derived from blood serum

Conference Name: CPD accredited 2nd International Conference on Molecular Biology & Stem Cells

Short Name : Molecular Biology 2019

Venue: London, UK | June 06-07, 2019

URL: https://goo.gl/Ew42oj

Mineral nanoparticles (NPs) are shown to create in human biological fluids once the concentration of Ca, carbonate and phosphate ions exceeds saturation. These mineral particles represent the primary mineral that forms in bones and teeth, likewise as in ectopic calcifications and excretory organ stones. The particles are detected within the body fluids and tissues of patients affected by numerous conditions, together with coronary artery disease, chronic renal disorder and kind two diabetes. As such, the particles represent precursors of physiological and pathological mineralization processes that occur within the human body.

Recent studies indicate that the mineral particles participate in different physiological roles in vivo. as an example, the particles form within the lumen of human intestines wherever they bind to dietary antigens and bacteria-derived molecules like peptidoglycan; the particles successively present these molecules to macrophages and nerve fiber cells of the intestinal mucous membrane to induce immunologic tolerance against food and therefore the gut microbiota. Similar mineral particles referred to as calciprotein particles (CPPs) have additionally been detected in busted foetal membranes from idiopathic preterm birth, suggesting that the particles might also have an effect on human fertility and foetal development.

We advised earlier that mineral particles forming within the human body might influence physiological processes by binding to numerous organic molecules in biological fluids. consequently, proteins bind to the particles and kind a “protein corona”, probably influencing the amount of these proteins in biological fluids. Identification of the molecules that bind to the mineral particles is required to assess the consequences of the particles’ organic parts in vivo. supported numerous techniques as well as biological staining, fluorescent tagging, ionophoresis, chromatography, genetics and metabolomics, we present here a comprehensive analysis of the organic corona of mineral particles formed in bovine and human blood serum.